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Looking for providers (Asia) for clone development and characterisation of cell line & expression

  • Contract manufacturing, filling and packaging, Pharmaceutical process development and validation, Analytical laboratory and testing services
  • Biopharmaceuticals
  • Asia
  • 04/10/2013

Description

We are looking for providers offering the following services (Indian or at least Asian providers preferred):

1. DNA Cloning (E. Coli & rCHO)- Clone genes of interest in vectors, amplification of genes, development of unique restriction sites.
2. Selection of cells that contain the gene (using different methods DHFR, resistance to antibiotics)
3. Custom Stable Cell Line Development Service: Isolation of cells originated from clones that have the characteristics of choice: high productivity of intact and active protein, stability of cell line
4. Propagation of cells and distribution to tubes under cGMP conditions
5. Storage of the cell bank (-70°C or liquid nitrogen as required)
6. Phenotypic characterization
7. Stability testing
8. qRTPCR Assays - Quantitative Real Time PCR assay development, probe and primer selection and running of assay.
9. Sequencing (Sanger or Next Generation Deep Sequencing)
10. SAGE (Serial Analysis of Gene Expression) Site-Directed Mutagenesis - Introduce mutations in gene or genome of interest such as deletions, insertions or point mutations

Requirements

  • Indian provider

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